Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation† †Electronic supplementary information (ESI) available: MS and LC-MS characterisation for thioester 2 and the M54L EPO mutant. See DOI: 10.1039/b811501j Click here for additional data file.

摘要

CNBr-mediated protein cleavage can result in a protein fragment containing an N-terminal cysteine. Fragments so produced are used in native chemical ligation reactions resulting in selectively modified biologically active proteins.